2024, Vol. 6, Special Issue 6, Part C

Nanotechnology is most active research area in the modern material science. Synthesis of silver nanoparticles (AgNPs) using plant green leaf extract is achieving popularity because of its environmental friendly nature and cost effective and also use of aqueous extract of leaves is for stabilization or reduction purpose. Nowadays, silver nanoparticles (AgNPs) are attracting attention due to their attractive properties, such as the good electrical and thermal conductivity, photo-electrochemical activity, chemical stability and high catalytic and antimicrobial and antioxidant activities. The characterization of silver nanoparticles, can be done by Uv-Vis spectrophotometer, whereas they can be detected by microscopic technique like TEM, SEM, XRD, FITR etc. The antimicrobial activity can be done with bioassay technique by employing gram positive and negative bacteria as test organism. The antioxidant activity can be done with bioassay technique by employing DPPH free radical and FRAP. M. oleifera extract was prepared with three different solvent distilled water, polar solvent (Methanol), Non-polar (hexane) and incubate for at least for 48 hours in incubator. Then the extract was filtered by using Whatmann paper and the crude extract is used for Phytochemicals screening, antioxidant assay and for synthesis of silver nanoparticles. The M. oleifera has a high content of phytonutrients therefore it is used to cure malnutrients as natural integrators. Phytochemicals such as Alkaloids, Saponins, Flavonoids, Terpenoids, Phenols are present in huge amount in M. oleifera leaf extract which have medicinal properties such as anti-carcinogenic, anti-diabetic, antioxidant, immunomodulatory, hepatoprotective and antiatherogenic. M. oleifera have been evaluated for its antioxidant activities. The antioxidant activity of the extract were evaluated by 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical scavenging activity assay. The synthesized silver nanoparticles also shown antioxidant activity. Their antioxidant activity was also evaluated by 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical scavenging activity assay. AgNPs are extensively used in many applications, mainly medical and biological applications. Synthesis of nanoparticles may serve as a future direction in biomedical nanotechnology in developing antimicrobial compounds, antioxidant compounds, treatment and prevention of diseases and significant advance in the diagnosis.